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Service Fees

Service bundle A. Mouse model generation services. Note this service is divided into four billable units 

1. Gene Editing Project Design and consultation*   $2,000 
2. Microinjection@   $5,000 
3. Reagents#   $1,000-4,000 
4. Genotyping of G0 offspring & custom breeding$  $1,000-3,000 

* The design service begins with an initial discussion to understand the client's project goals, followed by a detailed analysis of the target gene or locus, literature review and identification of potential genome editing strategies. The Core director and staff work collaboratively with the investigator’s team to evaluate and refine these options. This consultation continues throughout the project and beyond the initial phase, ensuring sustained support and success. 

@ Microinjection service is competitively priced at $5,000, which covers injections of up to 200 zygotes, or until at least one correctly targeted founder mouse is obtained, whichever comes first. 

#  Reagents cost largely depends on the length of the targeting DNA insert. Costs are cheaper for simple knockouts and short knock-ins, larger constructs cost more.  

$ Genotyping costs can vary significantly due to project-specific differences. Genotyping of founder mice generated using the CRISPR/Cas9 tool is particularly complex because of mosaicism and unpredictable DNA repair at target sites. The Core conducts initial on-target analysis using PCR (including overlapping primers) followed by Sanger sequencing. Germline transmission is confirmed in F1 offspring. We are also planning to expand our capabilities to include Long Read sequencing in collaboration with the Molecular and Genomics Core Facility. 

Service B. Embryological services

Embryo Cryopreservation of mutant line: Investigator supplies both male and female mice	$2500
Embryo Cryopreservation of heterozygous line: Investigator supplies males only	$3000
Sperm Cryopreservation	$750
Sperm Cryopreservation + IVF validation to test fertility	$1000
Sperm Cryopreservation + IVF and blastocyst genotype validation	$1250
Annual Cryostorage	$100
Rederivation from cryopreserved Sperm	$2500
Rederivation from cryopreserved Embryos	$1500

Service C: Custom Research Projects. Each project in this category is evaluated individually, with customized strategies developed in close collaboration with the investigator to meet specific research goals. A cost estimate is provided prior to project initiation, with the understanding that final costs may vary based on project outcomes. Core’s R&D Activities: The GREAT Core is actively involved in research and development to enhance and expand its service offerings. We currently employ proven methods such as Easi-CRISPR and GONAD to generate complete knockouts, conditional alleles and knock-in models with insert sizes up to 3 kb.Recognizing the current lack of robust methods for inserting larger genetic cassettes in core facility settings, the Core is investing in R&D to test and optimize new protocols. The goal is to transform these innovations into standardized, scalable services that broaden the capabilities available to our clients.